Osmosis is the diffusion of water from a dilute to a concentrated solution through a partially permeable membrane. Free notes and 13 questions answered.
Updated 20 Mar 2026 · Revise Right Team
Understanding Osmosis is part of Transport in cells in GCSE AQA Higher Biology.
3 min
Reading time
22
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GCSE AQA Higher
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What this lesson covers
Osmosis is the diffusion of water from a dilute to a concentrated solution through a partially permeable membrane. Free notes and 13 questions answered.
Define osmosis as the diffusion of water through a partially permeable membrane
Explain why water moves from dilute to concentrated solutions
Compare osmosis with simple diffusion
Apply knowledge of osmosis to predict water movement in cells
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Use this lesson to stay inside Transport in cells while connecting the detail back to the wider Cell biology topic.
Cell biology
Transport in cells
GCSE AQA Higher Biology
Revision notes
Read the core explanations from Understanding Osmosis before testing yourself from memory.
1. What Is Osmosis?
Picture two glasses of water separated by a special membrane. One glass has pure water, the other has sugar dissolved in it. Leave them overnight and something fascinating happens - water moves from the pure side to the sugary side, even though nothing is pushing it.
This movement has a name: osmosis. It's happening in your body right now - in every cell, keeping you alive.
Trap: Many students think osmosis needs energy or a pump. Actually, it's completely passive - water moves down its concentration gradient naturally, just like diffusion.
2. The Membrane That Makes It Happen
Osmosis can't happen through any old barrier. It needs a partially permeable membrane - think of it as a molecular bouncer that's very picky about who gets through.
Cell membranes are partially permeable. Water molecules are tiny and can pass through, but many solute particles cannot. Glucose and proteins are too large to pass through easily, and ions are also restricted by the membrane's selective properties.
This creates the perfect setup for osmosis. Water can move to balance things out, but the dissolved substances can't follow.
3. Why Water Moves - The Science Behind It
Here's the key insight: water doesn't actually 'want' to move anywhere. It's all about concentration gradients and random molecular motion.
In pure water, water molecules are bouncing around randomly. In sugar water, some of those bouncing spaces are taken up by sugar molecules. So there are fewer water molecules per unit volume - it's more dilute in terms of water.
Water molecules move randomly in all directions, but there's a net movement from where water is more concentrated (pure water) to where it's less concentrated (sugar water).
Trap: Students often think 'concentrated solution' means lots of water. Actually, a concentrated sugar solution has less water per unit volume because the sugar takes up space.
4. Osmosis in Living Cells
Now for the real-world application. Every cell in your body is surrounded by a partially permeable membrane, making osmosis crucial for life.
Plant cells have an advantage - their cell wall prevents them from bursting when water enters by osmosis. Animal cells aren't so lucky and can burst if they take in too much water.
Worked example: Predicting Osmosis in Cells
A plant cell with 5% sugar solution is placed in pure water. Predict what happens.
Inside cell: 5% sugar (concentrated)
Outside cell: Pure water (dilute) — The cell contents are more concentrated than the surrounding water
Water moves from dilute → concentrated
So water enters the cell — Water moves from a dilute solution to a more concentrated solution
Cell swells as water enters
Cell wall prevents bursting — Plant cells become turgid (firm) but don't burst due to the cell wall
Cell becomes turgid
Provides structural support to plant — This is how plants stay upright without bones!
Answer: Water enters the cell by osmosis, making it turgid and firm
5. Exam Technique - Getting Full Marks
Osmosis questions are exam favourites because they test both knowledge and application. Here's how to nail them every time.
Common exam mistake: Writing 'water moves to balance concentrations'. This is vague. Instead write: 'water moves from dilute solution to concentrated solution by osmosis through the partially permeable membrane'.
Master this process and you'll find osmosis questions become straightforward mark-winners. The key is understanding the direction of water movement and being able to explain it clearly using the correct scientific terminology.
In plant tissues, osmosis supports structure by maintaining turgor pressure in cells. In animal cells, there is no cell wall, so water movement can cause cells to burst in very dilute conditions or shrink in concentrated conditions. In exam answers, always state direction as water moving from dilute to concentrated solution through a partially permeable membrane.
Sources and content standards
Published by Revise Right. Use the official specification reference to check the requirements for your course. Content updated: 2026-03-20.
Osmosis is the diffusion of water through a partially permeable membrane from a dilute solution to a concentrated solution. It is completely passive: water moves down its concentration gradient naturally, just like diffusion, without needing energy or a pump.
What is the difference between osmosis and diffusion?
Osmosis is the diffusion of water through a partially permeable membrane. Like simple diffusion, it is completely passive and goes down a concentration gradient, but in osmosis water can move to balance things out while the dissolved substances can't follow.
What is a partially permeable membrane?
A partially permeable membrane lets tiny water molecules pass through, but many solute particles cannot. Cell membranes are partially permeable: glucose and proteins are too large to pass through easily, and ions are also restricted by the membrane's selective properties.
How does osmosis affect plant and animal cells?
In plant cells, the cell wall prevents bursting when water enters by osmosis, and osmosis maintains turgor pressure that supports plant structure. Animal cells have no cell wall, so water movement can make them burst in very dilute conditions or shrink in concentrated conditions.
What happens to a plant cell in pure water?
A plant cell in pure water takes in water by osmosis, because the cell contents are more concentrated than the surrounding water. The cell swells and becomes turgid, but the cell wall prevents it from bursting.
What happens to animal cells in a concentrated solution?
Animal cells in a concentrated solution shrink, because water moves out of the cell by osmosis into the more concentrated solution. Animal cells have no cell wall, so in very dilute conditions they can instead burst.
What does turgid mean?
Turgid means a plant cell is firm because water has entered it by osmosis. The cell wall prevents the cell from bursting, and turgid cells provide structural support, which is how plants stay upright without bones.
Does osmosis need energy?
Osmosis does not need energy or a pump; it is completely passive. Water moves down its concentration gradient naturally, from a dilute solution to a concentrated solution through a partially permeable membrane, just like diffusion.
Why do animal cells burst in pure water but plant cells do not?
Animal cells can burst in pure water because they have no cell wall, so they keep taking in water by osmosis. Plant cells also swell as water enters, but their cell wall prevents them from bursting, so they become turgid instead.
Does a concentrated solution have more water?
A concentrated solution has less water per unit volume, not more, because the dissolved substance, such as sugar, takes up space. So in osmosis water moves from a dilute solution, where water is more concentrated, to a concentrated solution.
Why can't sugar cross the membrane in osmosis?
Sugar cannot easily cross a partially permeable membrane because glucose molecules are too large, while water molecules are tiny and can pass through. Water can move to balance things out, but the dissolved substances cannot follow, which creates the setup for osmosis.
How should osmosis be described in an exam answer?
Osmosis should be explained as water moving from a dilute solution to a concentrated solution through a partially permeable membrane. Writing that 'water moves to balance concentrations' is too vague to earn credit.
Why does water move from a dilute to a concentrated solution?
Water moves from a dilute to a concentrated solution because a concentrated solution has fewer water molecules per unit volume. Water molecules move randomly in all directions, but there is a net movement from where water is more concentrated to where it is less concentrated.