Efficiency = (biomass at higher level ÷ biomass at lower level) × 100%. Free GCSE AQA Biology notes with key terms and 10 questions answered.
Updated 20 Mar 2026 · Revise Right Team
Calculating Biomass Transfer Efficiency is part of Trophic levels in an ecosystem (biology only) in GCSE AQA Higher Biology.
3 min
Reading time
36
Lessons in this topic
GCSE AQA Higher
Pathway
Reviewed by Revise Right
Content review
What this lesson covers
Efficiency = (biomass at higher level ÷ biomass at lower level) × 100%. Free GCSE AQA Biology notes with key terms and 10 questions answered.
Calculate biomass transfer efficiency between trophic levels using percentages or fractions
Explain why energy transfer efficiency affects the number of organisms at each level
Apply efficiency calculations to real ecosystem data
Evaluate the impact of low transfer efficiency on food chain length
Revise Right Mocks '26
AQA GCSE Biology mock draft, 26–31 Oct. Draft coverage: Biology Paper 1 (Higher tier). Reviewed for an original-practice sitting; check student availability before registering. Marking and results will follow later, after separate approval. Eligible new accounts receive a five-day Pro trial when signup email verification completes. Previous trials keep their original expiry. After expiry, new papers need Pro unless you have an explicitly confirmed free sitting offer for this event. That offer adds no Pro time or AI learning. Saved attempts and released results remain readable. No card or automatic charge.
Use this lesson to stay inside Trophic levels in an ecosystem (biology only) while connecting the detail back to the wider Ecology topic.
Ecology
Trophic levels in an ecosystem (biology only)
GCSE AQA Higher Biology
Revision notes
Read the core explanations from Calculating Biomass Transfer Efficiency before testing yourself from memory.
1. Why Only 10% Makes It Through
Picture a field of grass feeding rabbits, which feed foxes. You might expect thousands of grass plants to support hundreds of rabbits and dozens of foxes. But the numbers drop off much more dramatically than that. Why?
The answer lies in energy transfer efficiency. When a rabbit eats grass, it doesn't magically transform 100% of that plant material into rabbit biomass. Most of it gets lost along the way.
Trap: Many students think 'efficiency' means how good an animal is at hunting. Actually, it's about how much of the food eaten becomes new body mass. Even the best predators only convert about 10% of their prey's biomass into their own.
2. Where Does the Other 90% Go?
When a rabbit munches grass, here's what happens to that plant material:
Lost as heat (biggest chunk): The rabbit's metabolism generates heat to keep it warm. This energy can't be passed to the next level.
Lost in waste: Not all plant material gets digested. Rabbit droppings contain undigested cellulose that the rabbit couldn't break down.
Used for movement and life processes: Running from predators, breathing, and maintaining body functions all use energy that doesn't become biomass.
3. The Calculation Toolkit
You can also express this as a fraction instead of a percentage. An efficiency of 10% is the same as 0.1 or 1/10.
Worked example: Calculating Transfer Efficiency
In a pond ecosystem, 5000g of algae supports 400g of water fleas, which support 30g of small fish. Calculate the efficiency of transfer from algae to water fleas. [3 marks]
$$\text{Efficiency} = \frac{\text{Biomass at higher level}}{\text{Biomass at lower level}} \times 100\%$$ — [M1] for correct formula
Higher level (water fleas) = 400g
Lower level (algae) = 5000g — Water fleas eat algae, so they're the higher trophic level
$$\text{Efficiency} = \frac{400}{5000} \times 100\% = 0.08 \times 100\% = 8\%$$ — [M1] for substitution [A1] for correct answer
Answer: 8%
4. Why This Limits Food Chain Length
Here's where the maths gets dramatic. If only 10% transfers at each level, let's see what happens in a food chain:
Worked example: Biomass Through a Food Chain
Starting with 10,000g of grass, calculate the biomass at each trophic level assuming 10% efficiency.
Grass = 10,000g — This is our starting point - the primary producers
Notice how quickly the biomass drops off. By level 4, there's barely enough energy to support a single large predator. This is why food chains rarely exceed 4-5 trophic levels.
5. Exam Technique - Getting Full Marks
Efficiency calculations are mark-heavy questions. Here's how to secure every available mark:
Exam depth extension: In calculating biomass transfer efficiency, connect why only 10% makes it through to where does the other 90% go? using clear cause-and-effect statements. When you explain biological processes, include what changes, why it changes, and the consequence for cells, organisms, or populations. If calculations appear, show each step, include units, and end with a sentence that interprets the result in biological context.
Common mistake: Students often calculate what's lost (90%) instead of what transfers (10%). Always check your answer makes sense - efficiency should be a small percentage, not a large one.
Understanding biomass transfer efficiency explains why there are millions of grass plants, thousands of herbivores, but only a few top predators in any ecosystem. The maths is unforgiving - energy losses at each level create a pyramid that gets narrower and narrower until there's not enough energy left to support more levels.
Sources and content standards
Published by Revise Right. Use the official specification reference to check the requirements for your course. Content updated: 2026-03-20.
Efficiency = (biomass at higher level ÷ biomass at lower level) × 100%. If 5000g of algae supports 400g of water fleas, efficiency = (400 ÷ 5000) × 100% = 0.08 × 100% = 8%.
What is biomass transfer efficiency?
Biomass transfer efficiency is the percentage of biomass from one trophic level that becomes biomass in the next trophic level, usually around 10% in most ecosystems. It is not how good an animal is at hunting, but how much of the food eaten becomes new body mass.
Can transfer efficiency be written as a fraction?
Transfer efficiency can be expressed as a fraction instead of a percentage: an efficiency of 10% is the same as 0.1 or 1/10. Efficiency should be a small percentage, so calculating what is lost (90%) instead of what transfers is a common mistake.
How do you work out biomass at each trophic level?
To work out the next level, take the biomass at each level × the efficiency. Starting with 10,000g of grass at 10%: rabbits = 10,000 × 0.1 = 1,000g, foxes = 1,000 × 0.1 = 100g, and eagles = 100 × 0.1 = 10g.
Where does the 90% of biomass go?
The 90% of biomass that does not make it through is mostly lost as heat from metabolism, which keeps the animal warm; some is lost in waste, such as undigested cellulose in rabbit droppings; and some is used for movement and life processes like breathing.
Does efficiency mean how good an animal is at hunting?
Biomass transfer efficiency does not mean how good an animal is at hunting; it means how much of the food eaten becomes new body mass. Even the best predators only convert about 10% of their prey's biomass into their own.
What is the efficiency from algae to water fleas?
In a pond where 5000g of algae supports 400g of water fleas, the transfer efficiency is 400 ÷ 5000 × 100% = 8%. Water fleas eat algae, so they are the higher trophic level.
Why do rabbit droppings show biomass loss?
Rabbit droppings contain undigested cellulose that the rabbit could not break down, showing that not all plant material eaten becomes rabbit biomass. Biomass is also lost as heat and used for movement and life processes.
What is the biggest cause of biomass loss?
The biggest share of lost biomass goes as heat: a rabbit's metabolism generates heat to keep it warm, and this energy cannot be passed to the next level. The rest is lost in waste and used for movement and life processes.
How much biomass is left by trophic level 4?
Starting with 10,000g of grass and 10% efficiency, rabbits have 1,000g, foxes 100g and eagles only 10g. By level 4 there is barely enough to support a single large predator, which is why food chains rarely exceed 4-5 levels.